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cryo human hepatocytes  (Thermo Fisher)


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    Thermo Fisher cryo human hepatocytes
    Cryo Human Hepatocytes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cryo+human+hepatocytes/pmc11722105-56-0-4
    Average 90 stars, based on 1 article reviews
    cryo human hepatocytes - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: A prognostic molecular signature of hepatic steatosis is spatially heterogeneous and dynamic in human liver
    Article Snippet: Cryo Human Hepatocytes , Gibco , HU8305.

    Article Title: Methods and compositions relating to steroid hormone receptor-dependent proliferative disorders
    Article Snippet: 24 hours prior to treatment, cells were plated at 350,000 cells/well (700,000 cells/mL) in the following media: Williams E medium (WEM, A1217601), 2.5 mL FBS, 5 μL, 10 mM dexamethasone (from Gibco Thawing/Plating Supplement Pack, CM3000), 1.8 mL thawing/plating cocktail (from Gibco Thawing/Plating Supplement Pack, CM3000).

    Incubation:

    Article Title: O-GlcNAcylation enhances CPS1 catalytic efficiency for ammonia and promotes ureagenesis.
    Article Snippet: .. Mouse liver lysates (500 μg), insect cells lysates (500 μg), or commercially available human hepatocytes (Cryo Human Hepatocytes from GIBCO, Source: Single Donor, Lot# HU8300) (200 μg) were incubated with anti-CPS1 antibody (Abcam, Cat# ab45956) (ratio 1 μg antibody:100 μg lysate) overnight at 4 °C under rotation. ..



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    ( A ) RT-PCR of treated RH30 rhabdomyosarcoma, HeLa cervical cancer cells, H441 lung adenocarcinoma cells, and primary human <t>hepatocytes</t> following exposure to KTS morpholino or its inverted control. The upper 307-bp band indicates the presence of Exon 1 in the PCR amplicon, whereas the lower 185-bp PCR amplicon indicates exon skipping. We observed a dose-dependent induction of KRAS Exon 1 skipping following treatment with the morpholino, while KRAS Exon 2/3 and GAPDH housekeeping controls were not affected. ( B ) Immunoblots of KRAS and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein levels for the cell lines in (A). ( C ) KRAS expressed isoforms identified by PacBio Iso-Seq. 3′UTR, 3′ untranslated region. ( D to F ) KRAS isoforms detected in H441 cells exposed to KTS morpholino, inverted morpholino, or endoporter control. Isoforms displaying Exon 1 skipping are highlighted. Allele phasing by single nucleotide polymorphism database (dbSNP) represented by A (dbSNP containing) or B (lack of dbSNP) allele designation. ( G ) Percentage of circular consensus sequencing (CCS) reads that displayed KRAS Exon 1 skipping for each treatment group.
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    ( A ) RT-PCR of treated RH30 rhabdomyosarcoma, HeLa cervical cancer cells, H441 lung adenocarcinoma cells, and primary human hepatocytes following exposure to KTS morpholino or its inverted control. The upper 307-bp band indicates the presence of Exon 1 in the PCR amplicon, whereas the lower 185-bp PCR amplicon indicates exon skipping. We observed a dose-dependent induction of KRAS Exon 1 skipping following treatment with the morpholino, while KRAS Exon 2/3 and GAPDH housekeeping controls were not affected. ( B ) Immunoblots of KRAS and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein levels for the cell lines in (A). ( C ) KRAS expressed isoforms identified by PacBio Iso-Seq. 3′UTR, 3′ untranslated region. ( D to F ) KRAS isoforms detected in H441 cells exposed to KTS morpholino, inverted morpholino, or endoporter control. Isoforms displaying Exon 1 skipping are highlighted. Allele phasing by single nucleotide polymorphism database (dbSNP) represented by A (dbSNP containing) or B (lack of dbSNP) allele designation. ( G ) Percentage of circular consensus sequencing (CCS) reads that displayed KRAS Exon 1 skipping for each treatment group.

    Journal: Science Advances

    Article Title: Leveraging gene therapy to achieve long-term continuous or controllable expression of biotherapeutics

    doi: 10.1126/sciadv.abm1890

    Figure Lengend Snippet: ( A ) RT-PCR of treated RH30 rhabdomyosarcoma, HeLa cervical cancer cells, H441 lung adenocarcinoma cells, and primary human hepatocytes following exposure to KTS morpholino or its inverted control. The upper 307-bp band indicates the presence of Exon 1 in the PCR amplicon, whereas the lower 185-bp PCR amplicon indicates exon skipping. We observed a dose-dependent induction of KRAS Exon 1 skipping following treatment with the morpholino, while KRAS Exon 2/3 and GAPDH housekeeping controls were not affected. ( B ) Immunoblots of KRAS and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein levels for the cell lines in (A). ( C ) KRAS expressed isoforms identified by PacBio Iso-Seq. 3′UTR, 3′ untranslated region. ( D to F ) KRAS isoforms detected in H441 cells exposed to KTS morpholino, inverted morpholino, or endoporter control. Isoforms displaying Exon 1 skipping are highlighted. Allele phasing by single nucleotide polymorphism database (dbSNP) represented by A (dbSNP containing) or B (lack of dbSNP) allele designation. ( G ) Percentage of circular consensus sequencing (CCS) reads that displayed KRAS Exon 1 skipping for each treatment group.

    Article Snippet: Plateable cryo-preserved human hepatocytes (catalog no. HUCPG, Lonza Morristown, NJ USA) were thawed and transferred into 50 ml of warm thawing media (catalog no. MCHT50, Lonza) and spun down at 100 g for 8 min.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Amplification, Western Blot, Sequencing